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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
11/01/2021 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PEREIRA, M.; TISSOT, F.; FACCIO, R.; IBÁÑEZ, F.; PISTÓN, M. |
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Afiliación : |
MÓNICA PEREIRA, Universidad de la República, Facultad de Química, Montevideo, Uruguay.Grupo de Análisis de Elementos Traza y Desarrollo de Estrategias Simples para Preparación de Muestras (GATPREM).; FLORENCIA TISSOT, Universidad de la República, Facultad de Química, Montevideo, Uruguay.Grupo de Análisis de Elementos Traza y Desarrollo de Estrategias Simples para Preparación de Muestras (GATPREM).; RICARDO FACCIO, Universidad de la República, Montevideo, Uruguay. Facultad de Química. Área Física and Centro NanoMat.; FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIELA PISTÓN, Universidad de la República, Facultad de Química, Montevideo, Uruguay.Grupo de Análisis de Elementos Traza y Desarrollo de Estrategias Simples para Preparación de Muestras (GATPREM). |
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Título : |
A simple and economical ultrasound-assisted method for Cd and Pb extraction from fruits and vegetables for food safety assurance. [Open Access]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Results in Chemistry, Volume 3, Number 100089 2021, Doi: https://doi.org/10.1016/j.rechem.2020.100089 |
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DOI : |
10.1016/j.rechem.2020.100089 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 October 2020 / Accepted 16 December 2020 /
Corresponding author: mpiston@fq.edu.uy |
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Contenido : |
Ultrasound-assisted extraction (UAE) methods for food analysis are recognized as being in accordance with the principles of green analytical chemistry (GAC). Reference standard methods generally use microwave-assisted extraction (MAE) for sample digestion; however, the use of ultrasonication processes has many advantages. Herein, a simple, and economical method for the determination of Cd and Pb in fruits and vegetables for food safety monitoring is reported. This method was optimized using a standard reference material (SRM, spinach leaves) through a multivariate experimental design (optimal conditions: 0.5 g of sample, 15 min of sonication, and 20.00 mL of 2.5 mol L−1 HNO3) and subsequently validated. The limits of detection obtained were one or two orders of magnitude lower than those achieved with MAE, making this a promising technique for contaminants monitoring. The UAE method was then tested on vegetable samples (apples, carrots, tomatoes, and lettuce) obtained from commercial sources in Uruguay and a comparison was made with samples treated by MAE. Both processes yielded comparable results, and the levels of Cd and Pb in all samples were below the maximum allowed concentration (MAC) established by the World Health Organization (WHO). In addition, to provide insight into the mechanism by which the ultrasonic process aids extraction, confocal optical images of the surface of the SRM were obtained. The leaves were examined after treatment at different sonication times (5?20 min). The confocal microscopy images show the effects of the ultrasonic treatment on the plant tissue, including desaturation, erosion, and abrasion, which increased with increasing sonication time. In conclusion, the UAE method meets and overcome the requirements of a standard method for food safety assurance. MenosUltrasound-assisted extraction (UAE) methods for food analysis are recognized as being in accordance with the principles of green analytical chemistry (GAC). Reference standard methods generally use microwave-assisted extraction (MAE) for sample digestion; however, the use of ultrasonication processes has many advantages. Herein, a simple, and economical method for the determination of Cd and Pb in fruits and vegetables for food safety monitoring is reported. This method was optimized using a standard reference material (SRM, spinach leaves) through a multivariate experimental design (optimal conditions: 0.5 g of sample, 15 min of sonication, and 20.00 mL of 2.5 mol L−1 HNO3) and subsequently validated. The limits of detection obtained were one or two orders of magnitude lower than those achieved with MAE, making this a promising technique for contaminants monitoring. The UAE method was then tested on vegetable samples (apples, carrots, tomatoes, and lettuce) obtained from commercial sources in Uruguay and a comparison was made with samples treated by MAE. Both processes yielded comparable results, and the levels of Cd and Pb in all samples were below the maximum allowed concentration (MAC) established by the World Health Organization (WHO). In addition, to provide insight into the mechanism by which the ultrasonic process aids extraction, confocal optical images of the surface of the SRM were obtained. The leaves were examined after treatment at different sonication t... Presentar Todo |
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Palabras claves : |
CONFOCAL OPTICAL MICROSCOPY; FOOD SAFETY; PLATAFORMA AGROALIMENTOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14924/1/Results-in-Chemistry-2021-3-10089.pdf
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Marc : |
LEADER 02714naa a2200229 a 4500
001 1061662
005 2022-03-18
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.rechem.2020.100089$2DOI
100 1 $aPEREIRA, M.
245 $aA simple and economical ultrasound-assisted method for Cd and Pb extraction from fruits and vegetables for food safety assurance. [Open Access].$h[electronic resource]
260 $c2021
500 $aArticle history: Received 12 October 2020 / Accepted 16 December 2020 / Corresponding author: mpiston@fq.edu.uy
520 $aUltrasound-assisted extraction (UAE) methods for food analysis are recognized as being in accordance with the principles of green analytical chemistry (GAC). Reference standard methods generally use microwave-assisted extraction (MAE) for sample digestion; however, the use of ultrasonication processes has many advantages. Herein, a simple, and economical method for the determination of Cd and Pb in fruits and vegetables for food safety monitoring is reported. This method was optimized using a standard reference material (SRM, spinach leaves) through a multivariate experimental design (optimal conditions: 0.5 g of sample, 15 min of sonication, and 20.00 mL of 2.5 mol L−1 HNO3) and subsequently validated. The limits of detection obtained were one or two orders of magnitude lower than those achieved with MAE, making this a promising technique for contaminants monitoring. The UAE method was then tested on vegetable samples (apples, carrots, tomatoes, and lettuce) obtained from commercial sources in Uruguay and a comparison was made with samples treated by MAE. Both processes yielded comparable results, and the levels of Cd and Pb in all samples were below the maximum allowed concentration (MAC) established by the World Health Organization (WHO). In addition, to provide insight into the mechanism by which the ultrasonic process aids extraction, confocal optical images of the surface of the SRM were obtained. The leaves were examined after treatment at different sonication times (5?20 min). The confocal microscopy images show the effects of the ultrasonic treatment on the plant tissue, including desaturation, erosion, and abrasion, which increased with increasing sonication time. In conclusion, the UAE method meets and overcome the requirements of a standard method for food safety assurance.
653 $aCONFOCAL OPTICAL MICROSCOPY
653 $aFOOD SAFETY
653 $aPLATAFORMA AGROALIMENTOS
700 1 $aTISSOT, F.
700 1 $aFACCIO, R.
700 1 $aIBÁÑEZ, F.
700 1 $aPISTÓN, M.
773 $tResults in Chemistry, Volume 3, Number 100089 2021, Doi: https://doi.org/10.1016/j.rechem.2020.100089
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/09/2019 |
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Actualizado : |
23/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
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Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
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Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
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ISSN : |
1756-0500 |
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DOI : |
10.1186/s13104-015-1119-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published on: 16 April 2015. |
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Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
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Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
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Marc : |
LEADER 02324naa a2200277 a 4500
001 1060208
005 2019-09-23
008 2015 bl uuuu u00u1 u #d
022 $a1756-0500
024 7 $a10.1186/s13104-015-1119-7$2DOI
100 1 $aCOUTO, D.
245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource]
260 $c2015
500 $aArticle history: Published on: 16 April 2015.
520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central.
653 $aAffordable
653 $aArabidopsis
653 $aProtocol
653 $aRNA
653 $aTomato
653 $aWheat
700 1 $aSTRANSFELD, L.
700 1 $aARRUABARRENA, A.
700 1 $aZIPFEL, C.
700 1 $aLOZANO-DURÁN, R.
773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
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